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Alpha E catenin Antibody
Proteintech  
Product Name
Phoma pomorum var pomorum
Catalog Number
MYA-3682
Biosafety Level
1
Product Format
Frozen
Description
An ampoule containing viable cells suspended in cryoprotectant
Category
Fungi
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Human CPTP promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells 
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CPTP promotes growth and metastasis in PC cells. The effect of CPTP on PC (A) cell proliferation and (B) colony formation ability was determined using Cell Counting Kit-8 and colony formation assays, respectively. The effect of CPTP on migration and invasion were evaluated using Transwell and Matrigel assays in the (C) PANC-1 and (D) MIA PaCa-2 cell lines transfected with CPTP overexpression plasmid. (E) The relative protein expression levels of E-cadherin, <t>α-E-catenin,</t> β-catenin, Snail and vimentin were analyzed using Western blot analysis following CPTP overexpression. (F and G) Images of BALB/c-nude mice in each group 4 weeks after subcutaneous injection of CPTP overexpression or control vector in the PANC-1 cells. The tumor (H) weight and (I) volume from mice injected with CPTP overexpression vector cells compared with that in mice with empty vector cells. The PANC-1 cells stably overexpressing CPTP or the control vector (both 2x10 6 ) were subcutaneously injected into the right flank of nude mice. The tumor volume was calculated using the following equation: Volume = (length x width 2 )/2. The mice were injected with CPTP -overexpressing PANC-1 cells (5 × 10 6 /mouse) via tail vein to generate a metastasis model, representing H&E staining images of the lung tissues (J) and the number of metastatic nodules (K) in nude mice (n = 5 mice per group, scale bar = 100 μm). The lung metastatic nodules are indicated by the black arrows. *P < 0.05, **P < 0.01, ***P < 0.001. Ctrl, empty vector control; OE, overexpression.
Human CPTP promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells 
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CPTP knockdown suppresses growth and metastasis in PC cells. Cell proliferation and colony formation ability was assessed using a (A) Cell Counting Kit-8 assay and (B) colony formation assays in PC cells transfected with sh- CPTP or vector control (sh-NC). (C and D) Cell migration and invasion analysis following CPTP knockdown. (E) The relative protein expression levels of E-cadherin, <t>α-E-catenin,</t> β-catenin, Snail and vimentin were analyzed using Western blot analysis in CPTP knockdown cell lines. (F and G) Images of BALB/c-nude mice in each group 6 weeks after subcutaneous injection of CPTP knockdown or control vector PANC-1 cells. The tumor (H) weight and (I) volume in mice injected with sh- CPTP stable cells was compared with that in mice injected with vector control cells. The PANC-1 cells stably expressing sh- CPTP and control vector (sh-NC) (both 2x10 6 ) were subcutaneously injected into the right flank of BALB/c-nude mice. The tumor volume was calculated using the following equation: Volume = (length x width 2 )/2. For metastasis model establishment, CPTP -knockdown PANC-1 cells (5 × 10 6 /mouse) were injected into mice via tail vein (n = 5 mice per group, scale bar = 100 μm), representing pictures of lung tissues by H&E staining (J) and the number of metastatic nodules (K) in nude mice (n = 5 mice per group, scale bar = 100 μm). The lung metastatic nodules are indicated by the black arrows. *P < 0.05, **P < 0.01, ***P < 0.001. NC, negative control; sh, short hairpin.
Human CPTP promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells International Journal of Biological Sciences, 2022 Dec 14
"CPTP promotes growth and metastasis in PC cells. The effect of CPTP on PC (A) cell proliferation and (B) colony formation ability was determined using Cell Counting Kit-8 and colony formation assays, respectively. The effect of CPTP on migration and invasion were evaluated using Transwell and Matrigel assays in the (C) PANC-1 and (D) MIA PaCa-2 cell lines transfected with CPTP overexpression plasmid. (E) The relative protein expression levels of E-cadherin, <t>α-E-catenin,</t> β-catenin, Snail and vimentin were analyzed using Western blot analysis following CPTP overexpression. (F and G) Images of BALB/c-nude mice in each group 4 weeks after subcutaneous injection of CPTP overexpression or control vector in the PANC-1 cells. The tumor (H) weight and (I) volume from mice injected with CPTP overexpression vector cells compared with that in mice with empty vector cells. The PANC-1 cells stably overexpressing CPTP or the control vector (both 2x10 6 ) were subcutaneously injected into the right flank of nude mice. The tumor volume was calculated using the following equation: Volume = (length x width 2 )/2. The mice were injected with CPTP -overexpressing PANC-1 cells (5 × 10 6 /mouse) via tail vein to generate a metastasis model, representing H&E staining images of the lung tissues (J) and the number of metastatic nodules (K) in nude mice (n = 5 mice per group, scale bar = 100 μm). The lung metastatic nodules are indicated by the black arrows. *P < 0.05, **P < 0.01, ***P < 0.001. Ctrl, empty vector control; OE, overexpression. "
Techniques (20)
Western BlotWestern Blot30.66%
IncubationIncubation21.28%
StainingStaining6.35%
SDS PageSDS Page5.53%
Protocol Conditions
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