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"The following primary antibodies were used: Anti-CPTP (cat. No. HPA056832) from Atlas Antibodies, anti-Sp1 (cat. No. sc-17924X) and anti-Sp3 (cat. No. sc-28305X) from Santa Cruz Biotechnology, anti-E-cadherin (cat. No. 20874-1-AP), anti-α-E-catenin (cat. No. 12831-1-AP), anti-β-catenin (cat. No. 51067-2-AP), anti-Snail (cat. No. 13099-1-AP), anti-vimentin (cat. No. 10366-1-AP), anti-AKT (cat. No. 60203-2-IG), anti-phosphorylated (p)-AKT Ser473 (cat. No. 66444-1-AP) from ProteinTech Group (Wuhan, China), anti-p-AKT Thr308 (cat. No. 9275) and anti- KDM5B (JARID1B; cat. No. 3273) from Cell Signaling Technology, anti-PI4KA (cat. No. ab111565; Abcam), anti-SH3BP1 (cat. No. TA811580S) and anti-β-actin (cat. No. TA-09) from Origene Technologies."
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"Figure 2 CPTP promotes growth and metastasis in PC cells. The effect of CPTP on PC (A) cell proliferation and (B) colony formation ability was determined using Cell Counting Kit-8 and colony formation assays, respectively. The effect of CPTP on migration and invasion were evaluated using Transwell and Matrigel assays in the (C) PANC-1 and (D) MIA PaCa-2 cell lines transfected with CPTP overexpression plasmid. (E) The relative protein expression levels of E-cadherin, α-E-catenin, β-catenin, Snail and vimentin were analyzed using Western blot analysis following CPTP overexpression. (F and G) Images of BALB/c-nude mice in each group 4 weeks after subcutaneous injection of CPTP overexpression or control vector in the PANC-1 cells. The tumor (H) weight and (I) volume from mice injected with CPTP overexpression vector cells compared with that in mice with empty vector cells. The PANC-1 cells stably overexpressing CPTP or the control vector (both 2x10 6 ) were subcutaneously injected into the right flank of nude mice. The tumor volume was calculated using the following equation: Volume = (length x width 2 )/2. The mice were injected with CPTP -overexpressing PANC-1 cells (5 × 10 6 /mouse) via tail vein to generate a metastasis model, representing H&E staining images of the lung tissues (J) and the number of metastatic nodules (K) in nude mice (n = 5 mice per group, scale bar = 100 μm). The lung metastatic nodules are indicated by the black arrows. *P < 0.05, **P < 0.01, ***P < 0.001. Ctrl, empty vector control; OE, overexpression. "